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*Same as above, but show only results with accession number '''ENSP00000350063''': [http://db4.thegpm.org/thegpm-cgi/peak_search.pl?pmass=1735&pmrange=1&label=ENSP00000350063&submit=Search Results] | *Same as above, but show only results with accession number '''ENSP00000350063''': [http://db4.thegpm.org/thegpm-cgi/peak_search.pl?pmass=1735&pmrange=1&label=ENSP00000350063&submit=Search Results] | ||
*Parent ion mass '''889''' kDa, parent mass range of '''0.5''' kDa, fragment ion range of '''1.0''' kDa, filtered by sequence '''AMGIMNSFVNDIFER''' and accession number '''IPI00419833.7''', peaks '''ordered by intensity''' with only '''unique peaks''' shown: [http://db4.thegpm.org/thegpm-cgi/peak_search.pl?pmass=889&pmrange=.5&label=IPI00419833.7&seq=AMGIMNSFVNDIFER&unique=on&fmrange=1&sort=int&submit=Search Results] | *Parent ion mass '''889''' kDa, parent mass range of '''0.5''' kDa, fragment ion range of '''1.0''' kDa, filtered by sequence '''AMGIMNSFVNDIFER''' and accession number '''IPI00419833.7''', peaks '''ordered by intensity''' with only '''unique peaks''' shown: [http://db4.thegpm.org/thegpm-cgi/peak_search.pl?pmass=889&pmrange=.5&label=IPI00419833.7&seq=AMGIMNSFVNDIFER&unique=on&fmrange=1&sort=int&submit=Search Results] | ||
+ | |||
+ | ==References== | ||
+ | *Multiple Reaction Monitoring as a Method for Identifying Protein Posttranslational Modifications [http://jbt.abrf.org/cgi/content/abstract/16/2/83 Abstract] |
GPM-MRM is functionality that has been added to The GPM to assist researchers in finding signature combinations of peaks in MS/MS spectra. Spectra can be searched by any combination of parent ion mass, accession number or peptide sequence and the results can be pared down by fragment mass presence or uniqueness.
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The default values for some of the search parameters are as follows:
A search can be performed with any combination of the following parameters:
The order of precedence in searching is mass, residue sequence, accession number. If more than one of those parameters is provided, the lower priority parameters will be used to filter results that contain only that parameter.
For any of the above searches, providing the fragment ion mass will filter results that did not include a fragment ion within the given range of the supplied fragment ion mass.
The fragment ion mass range is used to classify fragment masses as unique or not. In a result set, and two masses between different results within the fragment ion mass range are considered to be the same, and therefore non-unique. A larger fragment ion mass range will lead to fewer fragment ions being considered unique.
The data returned by the search returns in the following order:
Unique fragment ion masses are colour-coded for fast identification, and the rows of results are ordered from fewest to most corresponding accession numbers. The peak/intensity tables can be displayed either by ascending weight or descending intensity. Non-unique peaks can be masked out for a simplified display.
The links below demonstrate the different search and display parameters (changeable parameters show in bold):