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*: it must have more than 8 but fewer than 31 residues; | *: it must have more than 8 but fewer than 31 residues; | ||
*: it cannot begin with Q, C, E, K, R or P; | *: it cannot begin with Q, C, E, K, R or P; | ||
- | * | + | *: it must end with K or R; |
*: it cannot contain an internal H, K or R; and | *: it cannot contain an internal H, K or R; and | ||
*: it cannot contain the sequence "NG". | *: it cannot contain the sequence "NG". |
The GPM's MRM database contains a listing of all of the peptide sequences and associated mass spectra associated with a particular protein sequence that may be needed to construct a multiple-reaction monitoring assay. This list is exhaustive: it contains many peptide sequences that may not be the best candidates for this type of assay. In order to simplify the task of assay development, we have developed system based on heuristics to reduce the number of candidate sequences.
The current set of heuristics for a candidate peptide sequence is as follows:
In addition, the observations must conform to the following rules:
Peptides that do not conform to these rules can be hidden or shown by clicking the link "show best candidates (*) only" on the main MRM display page for a protein.