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The Influence of Sample Preparation and Replicate Analyses on HeLa Cell Phosphoproteome Coverage. Bryan M. Ham, F Yang, H Jayachandran, N Jaitly, ME Monroe, MA Gritsenko, EA Livesay, R Zhao, SO Purvine, D Orton, J Adkins, DG Camp II, S Rossie, and RD Smith. Submitted to the Journal of Proteome Research in January 2008. The analyzed data can be viewed [http://gpmdb.thegpm.org/thegpm-cgi/plist.pl?npep=0&path=/gpm/archive/101/GPM10100151962.xml&proex=-1<ype=2 here]. | The Influence of Sample Preparation and Replicate Analyses on HeLa Cell Phosphoproteome Coverage. Bryan M. Ham, F Yang, H Jayachandran, N Jaitly, ME Monroe, MA Gritsenko, EA Livesay, R Zhao, SO Purvine, D Orton, J Adkins, DG Camp II, S Rossie, and RD Smith. Submitted to the Journal of Proteome Research in January 2008. The analyzed data can be viewed [http://gpmdb.thegpm.org/thegpm-cgi/plist.pl?npep=0&path=/gpm/archive/101/GPM10100151962.xml&proex=-1<ype=2 here]. | ||
| - | This data set was created from the merged MS/MS spectra used in this paper. The original data was obtained from the [http://ncrr.pnl.gov/data/#HomoSapiensData PNNL web site]. | + | |
| + | == Data source== This data set was created from the merged MS/MS spectra used in this paper. The original data was obtained from the [http://ncrr.pnl.gov/data/#HomoSapiensData PNNL web site]. Glutamic acid and asparatic acid residues have been methylated to reduce adventitious binding on a metal column. | ||
| + | |||
| + | ==Comments== The data is of good quality. It consists almost entirely of phosphopeptides. It also contains a significant number of peptide assignments that have not been previously observed. The observed proteins are predominantly nuclear and cytoplasmic. | ||
The Influence of Sample Preparation and Replicate Analyses on HeLa Cell Phosphoproteome Coverage. Bryan M. Ham, F Yang, H Jayachandran, N Jaitly, ME Monroe, MA Gritsenko, EA Livesay, R Zhao, SO Purvine, D Orton, J Adkins, DG Camp II, S Rossie, and RD Smith. Submitted to the Journal of Proteome Research in January 2008. The analyzed data can be viewed here.
== Data source== This data set was created from the merged MS/MS spectra used in this paper. The original data was obtained from the PNNL web site. Glutamic acid and asparatic acid residues have been methylated to reduce adventitious binding on a metal column.
==Comments== The data is of good quality. It consists almost entirely of phosphopeptides. It also contains a significant number of peptide assignments that have not been previously observed. The observed proteins are predominantly nuclear and cytoplasmic.