The Influence of Sample Preparation and Replicate Analyses on HeLa Cell Phosphoproteome Coverage. Bryan M. Ham, F Yang, H Jayachandran, N Jaitly, ME Monroe, MA Gritsenko, EA Livesay, R Zhao, SO Purvine, D Orton, J Adkins, DG Camp II, S Rossie, and RD Smith. Submitted to the Journal of Proteome Research in January 2008. The analyzed data can be viewed here.
This data set was created from the merged MS/MS spectra used in this paper. The original data was obtained from the PNNL web site. Glutamic acid and asparatic acid residues have been methylated to reduce adventitious binding on a metal column.
The data is of good quality. It consists almost entirely of phosphopeptides. It also contains a significant number of peptide assignments that have not been previously observed. The observed proteins are predominantly nuclear and cytoplasmic.