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- | Would it be a good idea to include the presence of Met in the heuristic rules for the exclusion of non-suitable peptides? I would be much more concerned about Met than about 3+ charged peptides (assuming that I can control for the latter through the inclusion of internal standards) | + | Would it be a good idea to include the presence of Met in the heuristic rules for the exclusion of non-suitable peptides? I would be much more concerned about Met than about 3+ charged peptides (assuming that I can control for the latter through the inclusion of internal standards). |
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+ | Another heuristic rule that I would find very sensible would be to exclude N-glycosylation sites NX[ST] - their presence would potentially mess up any SRM-based assay. |
Would it be a good idea to include the presence of Met in the heuristic rules for the exclusion of non-suitable peptides? I would be much more concerned about Met than about 3+ charged peptides (assuming that I can control for the latter through the inclusion of internal standards).
Another heuristic rule that I would find very sensible would be to exclude N-glycosylation sites NX[ST] - their presence would potentially mess up any SRM-based assay.